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sheep anti human nephrin igg  (R&D Systems)


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    R&D Systems sheep anti human nephrin igg
    Schematic overview of the workflow for the characterization of <t>anti-nephrin</t> autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.
    Sheep Anti Human Nephrin Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+anti+human+nephrin+igg/Human+Nephrin+Antibody/pmc12745956-81-8-12
    Average 95 stars, based on 110 article reviews
    sheep anti human nephrin igg - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity"

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

    Journal: Journal of Translational Autoimmunity

    doi: 10.1016/j.jtauto.2025.100307

    Schematic overview of the workflow for the characterization of anti-nephrin autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.
    Figure Legend Snippet: Schematic overview of the workflow for the characterization of anti-nephrin autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Pull Down Assay, Binding Assay

    Circulating antibodies against the extracellular domain of nephrin in idiopathic nephrotic syndrome (INS) patients . Panel A shows the levels of circulating anti-nephrin antibodies measured by ELISA in 10 healthy donors and 100 INS patients stratified based on biopsy. FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis. Additionally, in patients with FSGS and MCD, anti-nephrin levels were further stratified based on the type of INS. SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS. Panel B shows the titer of immunoprecipitating anti-nephrin antibodies in the serum and urine of the same subjects as in panel A. Panel C shows the prevalence of circulating anti-nephrin autoantibodies in the investigated cohorts of children and young adults with INS, with red and light gray colors representing positive and negative percentages, respectively. Panel D shows the prevalence of circulating anti-nephrin autoantibodies in FSGS and MCD samples stratified based on their pharmacological response. The dotted line indicates the statistically significant threshold.
    Figure Legend Snippet: Circulating antibodies against the extracellular domain of nephrin in idiopathic nephrotic syndrome (INS) patients . Panel A shows the levels of circulating anti-nephrin antibodies measured by ELISA in 10 healthy donors and 100 INS patients stratified based on biopsy. FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis. Additionally, in patients with FSGS and MCD, anti-nephrin levels were further stratified based on the type of INS. SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS. Panel B shows the titer of immunoprecipitating anti-nephrin antibodies in the serum and urine of the same subjects as in panel A. Panel C shows the prevalence of circulating anti-nephrin autoantibodies in the investigated cohorts of children and young adults with INS, with red and light gray colors representing positive and negative percentages, respectively. Panel D shows the prevalence of circulating anti-nephrin autoantibodies in FSGS and MCD samples stratified based on their pharmacological response. The dotted line indicates the statistically significant threshold.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Correlation between anti-nephrin antibody titers and urinary proteinuria in patients with anti-nephrin-associated MCD or FSGS . Panels A and B show the correlation between anti-nephrin antibody titers in serum, measured by ELISA (A) or immunoprecipitation (B), and 24-h proteinuria. Panels C and D show the corresponding correlation between urinary anti-nephrin antibody titers, measured by ELISA (C) or immunoprecipitation (D), and proteinuria. R = Spearman's correlation coefficient.
    Figure Legend Snippet: Correlation between anti-nephrin antibody titers and urinary proteinuria in patients with anti-nephrin-associated MCD or FSGS . Panels A and B show the correlation between anti-nephrin antibody titers in serum, measured by ELISA (A) or immunoprecipitation (B), and 24-h proteinuria. Panels C and D show the corresponding correlation between urinary anti-nephrin antibody titers, measured by ELISA (C) or immunoprecipitation (D), and proteinuria. R = Spearman's correlation coefficient.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunoprecipitation

    Evaluation of fucose in IgG by enzyme-linked immunosorbent assay (ELISA) with biotinylated lectins. Panels A and B show the levels of core-fucosylated IgG (α1,6-linked fucose) and antennary-fucosylated IgG (α1,2-linked fucose), respectively, measured using AAL and UEA-I lectins in the serum of healthy donors and NS patients, stratified by circulating anti-nephrin autoantibodies. Panels C and D display the inverse correlation between 24-h proteinuria and serum IgG fucosylation levels, as measured by AAL (Panel C) and UEA-I (Panel D), in patients with anti-nephrin–associated MCD or FSGS. Panels E and F show the corresponding levels of core and antennary fucosylation in IgG from urine samples, measured by AAL and UEA-I, and stratified by anti-nephrin antibody status. Red dots indicate patients positive for anti-nephrin autoantibodies, as confirmed by both ELISA and immunoprecipitation.
    Figure Legend Snippet: Evaluation of fucose in IgG by enzyme-linked immunosorbent assay (ELISA) with biotinylated lectins. Panels A and B show the levels of core-fucosylated IgG (α1,6-linked fucose) and antennary-fucosylated IgG (α1,2-linked fucose), respectively, measured using AAL and UEA-I lectins in the serum of healthy donors and NS patients, stratified by circulating anti-nephrin autoantibodies. Panels C and D display the inverse correlation between 24-h proteinuria and serum IgG fucosylation levels, as measured by AAL (Panel C) and UEA-I (Panel D), in patients with anti-nephrin–associated MCD or FSGS. Panels E and F show the corresponding levels of core and antennary fucosylation in IgG from urine samples, measured by AAL and UEA-I, and stratified by anti-nephrin antibody status. Red dots indicate patients positive for anti-nephrin autoantibodies, as confirmed by both ELISA and immunoprecipitation.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunoprecipitation

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity
    Article Snippet: After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.. Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.. Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.

    Pull Down Assay:

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity
    Article Snippet: After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.. Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.. Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.

    Binding Assay:

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity
    Article Snippet: After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.. Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.. Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.

    Immunoprecipitation:

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity
    Article Snippet: After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.After incubation, 5 μL of pre-washed Protein G Mag Sepharose Xtra beads (Merck Cat# GE28978118-AD) were added.. Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.. Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.Samples were incubated for 4 h at room temperature (RT) with shaking at 500 rpm to allow formation of IgG–nephrin complexes.



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    Schematic overview of the workflow for the characterization of <t>anti-nephrin</t> autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.
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    Schematic overview of the workflow for the characterization of anti-nephrin autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.

    Journal: Journal of Translational Autoimmunity

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

    doi: 10.1016/j.jtauto.2025.100307

    Figure Lengend Snippet: Schematic overview of the workflow for the characterization of anti-nephrin autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.

    Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Pull Down Assay, Binding Assay

    Circulating antibodies against the extracellular domain of nephrin in idiopathic nephrotic syndrome (INS) patients . Panel A shows the levels of circulating anti-nephrin antibodies measured by ELISA in 10 healthy donors and 100 INS patients stratified based on biopsy. FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis. Additionally, in patients with FSGS and MCD, anti-nephrin levels were further stratified based on the type of INS. SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS. Panel B shows the titer of immunoprecipitating anti-nephrin antibodies in the serum and urine of the same subjects as in panel A. Panel C shows the prevalence of circulating anti-nephrin autoantibodies in the investigated cohorts of children and young adults with INS, with red and light gray colors representing positive and negative percentages, respectively. Panel D shows the prevalence of circulating anti-nephrin autoantibodies in FSGS and MCD samples stratified based on their pharmacological response. The dotted line indicates the statistically significant threshold.

    Journal: Journal of Translational Autoimmunity

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

    doi: 10.1016/j.jtauto.2025.100307

    Figure Lengend Snippet: Circulating antibodies against the extracellular domain of nephrin in idiopathic nephrotic syndrome (INS) patients . Panel A shows the levels of circulating anti-nephrin antibodies measured by ELISA in 10 healthy donors and 100 INS patients stratified based on biopsy. FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis. Additionally, in patients with FSGS and MCD, anti-nephrin levels were further stratified based on the type of INS. SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS. Panel B shows the titer of immunoprecipitating anti-nephrin antibodies in the serum and urine of the same subjects as in panel A. Panel C shows the prevalence of circulating anti-nephrin autoantibodies in the investigated cohorts of children and young adults with INS, with red and light gray colors representing positive and negative percentages, respectively. Panel D shows the prevalence of circulating anti-nephrin autoantibodies in FSGS and MCD samples stratified based on their pharmacological response. The dotted line indicates the statistically significant threshold.

    Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay

    Correlation between anti-nephrin antibody titers and urinary proteinuria in patients with anti-nephrin-associated MCD or FSGS . Panels A and B show the correlation between anti-nephrin antibody titers in serum, measured by ELISA (A) or immunoprecipitation (B), and 24-h proteinuria. Panels C and D show the corresponding correlation between urinary anti-nephrin antibody titers, measured by ELISA (C) or immunoprecipitation (D), and proteinuria. R = Spearman's correlation coefficient.

    Journal: Journal of Translational Autoimmunity

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

    doi: 10.1016/j.jtauto.2025.100307

    Figure Lengend Snippet: Correlation between anti-nephrin antibody titers and urinary proteinuria in patients with anti-nephrin-associated MCD or FSGS . Panels A and B show the correlation between anti-nephrin antibody titers in serum, measured by ELISA (A) or immunoprecipitation (B), and 24-h proteinuria. Panels C and D show the corresponding correlation between urinary anti-nephrin antibody titers, measured by ELISA (C) or immunoprecipitation (D), and proteinuria. R = Spearman's correlation coefficient.

    Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunoprecipitation

    Evaluation of fucose in IgG by enzyme-linked immunosorbent assay (ELISA) with biotinylated lectins. Panels A and B show the levels of core-fucosylated IgG (α1,6-linked fucose) and antennary-fucosylated IgG (α1,2-linked fucose), respectively, measured using AAL and UEA-I lectins in the serum of healthy donors and NS patients, stratified by circulating anti-nephrin autoantibodies. Panels C and D display the inverse correlation between 24-h proteinuria and serum IgG fucosylation levels, as measured by AAL (Panel C) and UEA-I (Panel D), in patients with anti-nephrin–associated MCD or FSGS. Panels E and F show the corresponding levels of core and antennary fucosylation in IgG from urine samples, measured by AAL and UEA-I, and stratified by anti-nephrin antibody status. Red dots indicate patients positive for anti-nephrin autoantibodies, as confirmed by both ELISA and immunoprecipitation.

    Journal: Journal of Translational Autoimmunity

    Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

    doi: 10.1016/j.jtauto.2025.100307

    Figure Lengend Snippet: Evaluation of fucose in IgG by enzyme-linked immunosorbent assay (ELISA) with biotinylated lectins. Panels A and B show the levels of core-fucosylated IgG (α1,6-linked fucose) and antennary-fucosylated IgG (α1,2-linked fucose), respectively, measured using AAL and UEA-I lectins in the serum of healthy donors and NS patients, stratified by circulating anti-nephrin autoantibodies. Panels C and D display the inverse correlation between 24-h proteinuria and serum IgG fucosylation levels, as measured by AAL (Panel C) and UEA-I (Panel D), in patients with anti-nephrin–associated MCD or FSGS. Panels E and F show the corresponding levels of core and antennary fucosylation in IgG from urine samples, measured by AAL and UEA-I, and stratified by anti-nephrin antibody status. Red dots indicate patients positive for anti-nephrin autoantibodies, as confirmed by both ELISA and immunoprecipitation.

    Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunoprecipitation